Introduction

This experiment introduces a micro-volume protein quantification solution using
the POP UV-Vis Spectrophotometer from K LAB equipped with a Microvolume Cell
Holder, enabling the use of as little as 10 μL of protein sample for the Pierce™ 660
nm Protein Assay.
The POP is a standard UV-Vis Spectrophotometer widely used in laboratories and
offers flexible cell configurations to suit various analytical purposes, thanks to its
compatibility with multiple accessories. When equipped with the Microvolume Cell
Holder, it supports the use of disposable micro cuvettes in applications requiring
a minimum volume of only 70 μL, making it well-suited for analyzing small-volume
samples.
The Pierce™ 660 nm Protein Assay is a colorimetric method that quantifies protein
concentration by measuring the absorbance at 660 nm of a blue-colored complex
formed through the reaction between the protein and the assay reagent.