UV-Vis

ADMI and ASTA are standardized UV-Vis methods for quantifying color in wastewater and red spices using defined absorbance measurements.

Stray light is out-of-band radiation reaching the detector and is the dominant source of error at high absorbance in UV-Vis spectrophotometers.

Xenon flash lamps deliver intense broadband light in microsecond pulses, enabling stable low-noise measurements in modern spectrophotometers.

A monochromator isolates a narrow band of wavelengths from broadband light; the Czerny-Turner layout is the industry standard for compact UV-Vis instruments.

Diffraction gratings use microscopic periodic grooves to disperse light into its component wavelengths, forming the heart of modern spectrophotometers.

Cuvette material and path length determine which wavelengths can be measured and set the practical concentration range for any UV-Vis assay.

Microvolume spectrophotometers measure as little as 1 uL by compressing a liquid drop into an ultra-thin optical film between two surfaces.

Measuring absorbance at 260 nm and 280 nm gives both concentration and purity of DNA and RNA samples in a single rapid scan.

Bradford, BCA, Biuret, and Lowry assays each convert protein concentration into a measurable color change through different chemical mechanisms and working ranges.

ELISA uses enzyme-linked antibodies to detect and quantify proteins in solution, converting binding events into a measurable color change read by a microplate reader.